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5-Aminoallyl-2'-deoxyuridine-5'-Triphosphate (Aminoallyl-dUTP)
Features: ideal for enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random-primed labeling, or primer extension.
Formula: C12H20N3O14P3 (acid form)
Molecular weight: 523.2 (acid form)
Purity: > 96% (HPLC)
Concentration 100 mM
Description
Aminoallyl-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, Klenow Fragment, exo- and DNA Polymerase I. The resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten.
Applications
Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random-primed labeling, or primer extension.Storage | -20°C |