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7-deaza-dGTP (7-deaza-2'-deoxyguanosine-5'-triphosphate)
Features: 7-deaza dGTP may be used in sequencing for the resolution of the compressions in G-C-rich regions.
Description
7-deaza-dGTP may be effectively incorporated into the DNA by DNA polymerases and/or terminal deoxynucleotidyl transferase. 7-deaza-dG - containing DNA duplexes demonstrate increased stability, 7-deaza-dG - containing DNA penetrates cell membrane more effectively due to the increased lypophility.
7-deaza dGTP may be used in sequencing for the resolution of the compressions in G-C-rich regions. Amplification of some DNA regions is often hindered due to the high G-C content. Replacement of dGTP to 7-deaza dGTP in the master mix may solve the problem.
Delivery form Sterile water solution of ammonium salt nucleotide. Colorless transparent liquid.
Concentration 10 mM
Quality control - Purity according to HPLC analysis is not less than 96%.
- Product is free of DNases, RNases and phosphatases activities.
- The concentration is determined spectrophotometrically, λmax = 259 nm.
- pH = 5.0-5.5
Formula | C11H13N4O13P3 |
Molecular weight | 502.16 g/mol |
Storage | -20°C. Avoid multiple freezing-thawing. |
Transportation | The product does not require special conditions of transportation. |